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KMID : 0356920080540030328
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Korean Journal of Anesthesiology
2008 Volume.54 No. 3 p.328 ~ p.334
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Expression of Spinal c-fos in a Rat Model of Postoperative Pain
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Doh Sok-Ju
Choi Suk-Ki
Jin Hee-Chul
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Abstract
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Background :The oncogene, c-fos, is a useful marker of nociceptive neurons activated by various types of pain.However, the relationship between c-fos and acute pain induced by surgical incision has not been studied.The purpose of this study was to describe the expression of spinal c-fos in a rat model of postoperative pain.
Methods :Sprague-Dawley rats were used to enumerate spinal fos-like immunoreactive (fos-LI)-positive neurons after making sham, skin, or skin-muscle incisions on the plantar surface of the rat hindpaw. The spinal cords were divided into the following 4 segments for immunohistochemical detection of fos-LI at 3 time points after the incision (4 hours, POD1, and POD3):thoracic, upper lumbar, lower lumbar, and sacral.For analysis of the laminar distribution of fos-LI, the dorsal horn was divided into three regions (I?II, III?IV, and V?VI).
Results :The number of fos-LI was increased in the ipsilateral spinal dorsal horn in the lower lumbar segment 4 hours post-incision and was significantly greater in rats with skin-muscle incisions than in rats with skin-alone incisions.In the skin-muscle group, laminae I?II and V?VI had a greater number of fos-LI neurons than laminae III?IV.The increase in number of spinal fos-LI neurons normalized by POD1 and POD3.
Conclusion :c-fos expression is confined to neurons of the spinal dorsal horn known to be activated by nociceptive stimulation. The larger the injury elicited, the greater the expression of c-fos, suggesting that the expression of c-fos can serve as a useful marker of activated nociceptive neurons caused by acute pain following surgical incision.Further study is required to elucidate why the degree of tissue injury affects the laminar distribution of c-fos expression.(Korean J Anesthesiol 2008; 54: 328∼34)
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KEYWORD
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c-fos, postoperative pain, spinal
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